Identification and characterization of FGF2-dependent mRNA:microRNA networks during lens fiber cell differentiation

ABSTRACT: Background: FGF signaling controls numerous processes during cell lineage specification, organogenesis and terminal differentiation. In lens, FGF signaling was implicated as the key pathway that controls lens fiber cell differentiation, but little is known about its full range and spectrum of regulated genes. Results: Herein, we employed rat lens epithelial explant system and performed RNA and microRNA expression profiling in cells induced to differentiate by FGF2. The primary data were collected at explants grown overnight in the presence of 5 ng/ml of FGF2, followed by a treatment with 100 ng/ml of FGF2 and collection of samples at 2, 4, 12 and 24 hours. Global analysis identified extensive FGF2-regulated cellular responses that were both independent and dependent on microRNAs (miRNAs).

ORGANISM(S): Rattus norvegicus

SUBMITTER: Jiri Zavadil 

PROVIDER: E-GEOD-50604 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress