Targeted DNA demethylation using TALE-TET1 fusion proteins

ABSTRACT: Recent large-scale studies have defined genomewide, cell type-specific patterns of DNA methylation, a modification known to be important for regulating gene expression in both normal development and disease states. However, determining the functional significance of specific methylation events remains a challenging problem due to the current lack of targeted methodologies for removing these modifications. Here we describe an approach for efficient targeted demethylation of specific CpGs in human cells using fusions of engineered transcription activator-like effector (TALE) repeat arrays and the TET1 hydroxylase catalytic domain. Using these TALE-TET1 fusions, we demonstrate that modification of certain critical methylated promoter CpG positions can be associated with substantial increas

ORGANISM(S): Homo sapiens

SUBMITTER: Morgan Maeder 

PROVIDER: E-GEOD-50761 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress