Transcriptomes of isolated rice gametes characterized by deep sequencing: Evidence for distinct sex-dependent chromatin and epigenetic states before fertilization

ABSTRACT: The formation of a zygote by the fusion of egg and sperm involves the two gametic transcriptomes. In flowering plants, the embryo sac embedded within the ovule contains the egg cell, while the pollen grain contains two sperm cells inside a supporting vegetative cell. The difficulties of collecting isolated gametes and consequent low recovery of RNA have restricted in-depth analysis of gametic transcriptomes in flowering plants. We isolated living egg cells, sperm cells, and pollen vegetative cells from rice, and identified transcripts for ~36,000 genes by deep sequencing. The three transcriptomes are highly divergent, with about three quarters of those genes differentially expressed in the different cell types. Distinctive expression profiles were observed for genes involved in chromati

ORGANISM(S): Oryza sativa Japonica Group

SUBMITTER: Cameron Johnson 

PROVIDER: E-GEOD-50777 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress