Epithelial Ovarian Cancer Cells: Control vs. miR-506 Transfected
Ontology highlight
ABSTRACT: Transcriptional profiling of untreated ovarian cancer cells and ovarian cancer cells miR-506 transfected with 48hours. Two-condition experiment, control vs. miR-506 treated cells. One replicate per array.
Project description:Design a human-mouse dual-species microarray to provide a technology for investigation of cancer-stromal interaction in xenograft model. Reference RNA from human and mouse were labeled using Cy5, while the mixture of human and mouse RNA were labeled using Cy3. Samples were hybridized on customized-commercial array with Agilent probes and user-designed species-specific probes (Human-mouse dual-species array HomoMusArray v1).
Project description:Design a human-mouse dual-species microarray to provide a technology for investigation of cancer-stromal interaction in xenograft model. The 2 reference RNA were used to evaluate and identify probes with potential the cross-species hybridization, so that we can label these probes and exclude them before analysis of biological samples/data. Reference RNA from human and mouse were labeled using Cy3. Samples were hybridized on customized-commercial array with Agilent probes and user-designed species-specific probes (Human-mouse dual-species array HomoMus_v2). This array is designed and improved from hte first version GPL10714
Project description:This SuperSeries is composed of the following subset Series: GSE23054: Dual-species microarray for assessing gene expression in stromal microenvironment, cancer cells and their interactions (v1 array) GSE23364: Dual-species microarray for assessing gene expression in stromal microenvironment, cancer cells and their interactions (v2 array) Refer to individual Series
Project description:Transcriptional profiling of E.coli SE15 comparing wild type E.coli SE15 with Autoindecur 2 synthesis gene LuxS mutnat E.coli SE15. E.coli SE15 is isolated from indwelling catheter of urinary tract infected patient. Examine change of quorum sensing related gene by deleting autoinducer 2 synthesis gene LuxS in E.coli One array: Wild type E.coli SE15 vs. LuxS mutant E.coli SE15
Project description:Transcriptional profiling of P. putida KT2440 cells comparing control untreated wild type cells with untreated recG gene mutant cells or PQ treated recG gene mutant cells Three-condition experiment, Control vs. untreated recG gene mutant and PQ treated recG gene mutant. Biological replicates: 4 samples independently grown and harvested
Project description:Using the HL60 multipotent promyelocytic leukemia cell line, we performed experiments that lead to two different cell fate attractors by treatments of varying dosage and duration of the differentiation agent all-trans-retinoic acid (ATRA). The dosage and duration combinations corresponding to the two treatments were chosen by means of of cytometric measurements of CD11b, a well-known early differentiation marker, such that the two populations are poised at the same stage of differentiation. Using the selected treatment combinations, one population proceeds toward the terminally differentiated neutrophil attractor while the other population reverts back toward the undifferentiated promyelocytic attractor. We monitored the gene expression changes in the two populations after their respective treatments over a period of five days and identified a set of genes that diverged in their expression; a subset of which promotes neutrophil differentiation while the other represses cell cycle progression. By employing promoter based transcription factor binding site analysis, we found enrichment of transcription factors functionally linked to tumor progression, cell cycle, and development. Keywords: Time course, dose response Untreated HL60 cells are hybrized on the channel 1 with Cy3, ATRA-Treated HL60 cells are hybridized on channel 2 with Cy5. HK60 cells were treated with high dosage/short duration and low dosage/long duration to achieve 55% of CD11b+ cells. These positive cells were then isolated and culture in fresh media and total RNA were isolated for comparison. Cells treated with high dosage/short duration treatment proceed toward the neutrophil attractor while cells treated with the low dosage/long duration treatment revert back toward the promyelocyte attractor.
Project description:Transcriptional profiling of P. putida KT2440 cells comparing control untreated cells with PQ or CHP treated cells Three-condition experiment, Control vs. treatment. Biological replicates: 1 control, 3 treated, independently grown and harvested
Project description:Transcriptional profiling of mouse liver tissues comparing Wild type liver tissues with Wip1 KO mice liver tissues after Partial Hepatectomy at 24h and 36 h. WT vs. Wip1 KO tissues after Partial Hepatectomy at 24h and 36 h .