Comparison of gene expression in M-NM-^TrpaA strain and wild-type strain

ABSTRACT: The goal of the experiment was to determine the difference in gene expression between the wild-type strain and a strain lacking rpaA (M-NM-^TrpaA). Because gene expression is not at steady-state in the wild-type -- it oscillates with a circadian period -- and we did not know a priori whether it is at steady-state in the M-NM-^TrpaA strain, we compared the time-averaged gene expression in the wild-type to the time-averaged gene expression in the M-NM-^TrpaA strain. Cultures were grown in a turbidostat as described previously (Vijayan et al, PNAS 2009). Cultures were entrained with two consecutive light/dark cycles and released into continuous light at time T = 0. Cultures were samples every 4 hours for 20 h between T = 24 h and T = 44 h, inclusive (no 4 h sample was acquired because, in

ORGANISM(S): Synechococcus elongatus PCC 7942

SUBMITTER: Joseph Markson 

PROVIDER: E-GEOD-50919 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress