Identification of the alternative sigma factor SigX regulon and its implications for Pseudomonas aeruginosa pathogenicity

ABSTRACT: We analyzed a deletion mutant of the ECF M-OM-^C factor SigX and applied mRNA profiling to define the SigX dependent regulon in P. aeruginosa in response to low osmolarity medium conditions. Furthermore, the combination of transcriptional data with chromatin immunoprecipitation (ChIP) followed by high-throughput sequencing led to the identification of the DNA binding motif of SigX. Genome-wide mapping of SigX-binding regions revealed enrichment of downstream genes involved in fatty acid biosynthesis, type III secretion, swarming and c-di-GMP signaling. In accordance, a sigX deletion mutant exhibited an altered fatty acid composition of the cell membrane, reduced cytotoxicity, impaired swarming activity, elevated c-di-GMP levels and increased biofilm formation. In conclusion, a combination

ORGANISM(S): Pseudomonas aeruginosa UCBPP-PA14

SUBMITTER: Denitsa Eckweiler 

PROVIDER: E-GEOD-50937 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress