Comparative genomics reveals new molecular targets for accelerated strain improvement of rifamycin B-producing strains

ABSTRACT: In the present study, we have used a genomic approach to understand how the classical mutate-and-screen method actually generated an improved rifamycin B producer. Compared with the reference strains Amycolatopsis mediterranei S699 (rifamycin B producer) and U32 (rifamycin SV producer), a total of 250 variations affecting a total of 227 coding sequences (CDS) were found in rifamycin B overproducing strain HP-130. One hundred nine CDS variations were specific to HP-130 as they were absent in both S699 and U32. A lot of variations affected genes coding for fatty acid (an lipid) metabolism, which is tightly interconnected with rifamycin biosynthesis by common metabolic precursors (malonyl-CoA, methylmalonyl-CoA). Interestingly, a nonsense mutation was mapped within mutB coding for methylmalon

ORGANISM(S): Amycolatopsis mediterranei S699

SUBMITTER: Silvio Bicciato 

PROVIDER: E-GEOD-51015 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress