Gene expression profiles of E8.5, E9.5 and E11.5 kidney precursors

ABSTRACT: Understanding processes how the early stage kidney precursor gives rise to metanephric mesenchyme, which is a committed progenitor cells of adult kidney is important for the regeneration of kidney in vitro. The combination of fluorescent activated cell sorting (FACS) plus microarray analysis offers a powerful, efficient and effective method for the creation of global gene expression profiles of the developing kidney precursors. Those gene expression data provides insights into not only the stage specific marker genes but also the signals working in each population, which should be informative for the directed differentiation of pluripotent stem cells in vitro. Osr1-GFP knock-in mice were used to isolate kidney precursor cells from embryos at E8.5, E9.5 and E11.5. At E9.5 and E11.5 embryos,

ORGANISM(S): Mus musculus

SUBMITTER: Atsuhiro Taguchi 

PROVIDER: E-GEOD-51054 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress