ChIP-Seq analysis of RpaA binding dynamics

ABSTRACT: The response regulator RpaA is required for control of genome-wide gene expression by the cyanobacterial circadian clock. RpaA is predicted to be a DNA binding protein based on sequence homology, but prior studies have been unable to detect binding in vitro or in vivo to a small panel of promoters. We used ChIP-Seq to determine whether RpaA associates with DNA in vivo, and if so, with what dynamics. We find that RpaA binds to over 100 location in the genome in a circadian manner, with strongest binding occuring around subjective dusk. Analysis of these binding sites shows that RpaA directly regulates the expression of clock components to generate feedback on the core oscillator, and also regulates expression of a small set of circadian effectors that in turn orchestrate global expressi

ORGANISM(S): Synechococcus elongatus PCC 7942

SUBMITTER: Joseph Markson 

PROVIDER: E-GEOD-51093 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress