The Gene Expression Profile of Peripheral Blood Mononuclear Cells from EV71-Infected Rhesus Neonates

ABSTRACT: To study the gene expression during EV71 infection, we have employed whole Rhesus Monkey genome microarray expression profiling as a discovery platform to identify genes with the potential to distinguish infection process. Rhesus monkey peripheral blood from the experiment or control donors was isolated, extracted the RNA and screened by microarray. The results indicated that the expression levels of more than 500 functional genes associated with multiple pathways were modulated. To confirm the results of the microarray analysis, 25 individual genes were random selected and were analyzed using real-time RT-PCR amplification. The expression levels of genes associated with immune inflammatory responses were up-regulated during the period from day 4 to 10 post-infection. The expressions of tw

ORGANISM(S): Macaca mulatta

SUBMITTER: Ying Zhang 

PROVIDER: E-GEOD-51103 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress