Androgen receptor uses relaxed response element stringency for selective chromatin binding and transcriptional regulation in vivo

ABSTRACT: We report the in vivo androgen receptor (AR) binding sites in caput epididymis of intact SPARKI and wild-type mice using ChIP-sequencing. SPARKI (specificity affecting androgen receptor knock-in) mouse line has the second zinc finger of AR replaced by that of glucocorticoid receptors. In vivo analysis of SPARKI and wild-type AR genome-wide binding sites identified cis-element with less stringent sequence requirements that specify selective AR-binding sites. The ChIP experiments have been performed using antibody specific to AR and IgG non-specific antibody as a negative control. Examination of AR binding sites in epididymis of wild-type and SPARKI mice using ChIP-seq. Two biological replicates from intact wild-type and SPARKI mice and two control IgG samples were sequenced and used in peak calling. To increase the depth of the analysis, replicate ChIP-seq samples were merged and the concatanated samples were used in peak calling.

ORGANISM(S): Mus musculus

SUBMITTER: Olli Jänne 

PROVIDER: E-GEOD-51106 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress