Identification of building principles of methylation states at CG rich regions by high-throughput editing of a mammalian genome

ABSTRACT: Methylation is a repressive modification of DNA prevalent throughout mammalian genomes yet mostly absent at CG rich stretches referred to as CGI. Here we identify their building principles by parallel genomic targeting of sequence libraries. Iterative insertions generated over 3,000 variants of genome-derived and artificial sequences at the same genomic site. Single molecule profiling of the methylation status of this collection allowed modeling the contribution of CG content and DNA binding factors towards the unmethylated state. It made the surprising prediction that the majority of CGs within endogenous islands are susceptible to methylation changes modulated by the presence of transcription factors, which is indeed confirmed by genome-wide methylation dynamics during multiple cellula

ORGANISM(S): Mus musculus

SUBMITTER: Dirk Schuebeler 

PROVIDER: E-GEOD-51170 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress