Isolation of human iPSC-derived dopaminergic progenitors by cell sorting

ABSTRACT: Human induced pluripotent stem cells (iPSCs) can provide a promising source of midbrain dopaminergic (DA) neurons for cell replacement therapy for Parkinson’s disease. However, iPSC-derived donor cells may inevitably contain tumorigenic or inappropriate cells. Purification of neural progenitor cells or DA neurons as suitable donor cells has been attempted, but the isolation of DA progenitor cells derived from human pluripotent stem cells has so far been unsuccessful. Here we show human iPSC-derived DA progenitor cells can be efficiently isolated by cell sorting using a floor plate marker, Corin. we were able to develop a method for 1) scalable DA neuron induction on human laminin fragment and 2) sorting DA progenitor cells using an anti-Corin antibody. Furthermore, we determined the opti

ORGANISM(S): Homo sapiens

SUBMITTER: Daisuke Doi 

PROVIDER: E-GEOD-51214 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress