Dysregulation of genome-wide gene expression and DNA methylation in abnormal cloned piglets (RNA-Seq)

ABSTRACT: Backgroud:Epigenetic modifications (especially altered DNA methylation) resulting in altered gene expression may be one reason for development failure or the abnormality of the cloned animals, but the underlying mechanism of the abnormal phenotype in the cloned piglets remains unrevealed. Some cloned piglets in our study showed abnormal phenotypes such as big tongue (longer and thicker), limp, and exomphalos, which is similar to the human BWS syndrome. Here we conducted DNA methylation (DNAm) immunoprecipitation binding high throughput sequencing (MeDIP-seq) and RNA sequencing (RNA-seq) of muscle tissues of cloned piglets to investigate the relationship of abnormal DNAm with gene dysregulation and the unusual phenotypes in cloned piglets. Results:Analysis of the methylomes revealed that ab

ORGANISM(S): Sus scrofa

SUBMITTER: Changchun Li Li 

PROVIDER: E-GEOD-51282 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress