Assessment of hematopoietic failure due to RPL11 deficiency in a zebrafish model of DiamondM-bM-^@M-^SBlackfan anemia by deep sequencing

ABSTRACT: To comprehensively reflect the impact of RPL11 deficiency on the transcriptome of zebrafish embryos, we collected 40M-bM-^@M-^S50 RPL11-deficient and MO control zebrafish embryos at 48 hpf from separate experiments and constructed two mRNA-seq sequencing libraries in parallel. High-throughput sequencing was performed on the Hi-Seq2000 sequencing platform in parallel. The number of sequenced gene transcript reads was 35M-bM-^@M-^S40 million. We found that hemoglobin biosynthetic and hematological defects in RPL11-deficient zebrafish were related to dysregulation of iron metabolism-related genes, including tfa, tfr1b, alas2 and slc25a37, which are involved in heme and hemoglobin biosynthesis. In addition, we found reduced expression of the hematopoietic stem cells (HSC) marker c-myb and HSC

ORGANISM(S): Danio rerio

SUBMITTER: yadong yang 

PROVIDER: E-GEOD-51326 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress