Effect of ARF on the transcription of p53 target genes in homologous recombination deficient cells

ABSTRACT: ARF is a tumour suppressor activated by oncogenic stress, which stabilizes p53. Although p53 is a key component of the response to DNA damage, a similar function for ARF has not been ascribed. In this work we show that homologous recombination (HR) -deficient primary mouse and human cells accumulate DNA damage, which triggers checkpoint signalling and ARF activation. We also show that in the absence of ARF p53 is induce in response to DNA damage, but surprisingly fails to trigger senescence. The hypothesis tested in this study was that ARF deficiency alters the spectrum of genes activated by p53 in response to HR deficiency. Using mRNA microarray analysis and expression profiling, we identified genes upregulated in wild type primary MEFs treated with RAD51 shRNA, whose expression was not i

ORGANISM(S): Mus musculus

SUBMITTER: Ana Carlos 

PROVIDER: E-GEOD-51354 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress