High resolution mapping of modified nucleobases in DNA using excision repair enzymes

ABSTRACT: Data from Excision-seq experiments to map deoxyuridine and pyrimidine dimers in S. cerevisiae and E. coli For uracil mapping in pre-digestion Excision-seq, uracil was excised from genomic DNA from dut ung yeast and bacteria yielding double-stranded DNA fragments. Adapters were ligated to these fragments for Illumina library preparation. Using this method, the number of reads at a genomic location corresponds to the quantity of dU at that location. In post-digestion Excision-seq, uracil-containing library fragments are destroyed by UDG treatment, thus coverage is inversely proportional to uracil content. For pyrimidine dimer Excision-seq, yeast were irradiated with high doses of UVC light to generate a large number of DNA modifications.  Using the excision repair enzyme UVDE from S. pombe,

ORGANISM(S): Escherichia coli

SUBMITTER: Jay Hesselberth 

PROVIDER: E-GEOD-51361 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress