Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Comparison of microRNA Profiling Platforms (HTS)

ABSTRACT: Global miRNA expression profiling of human malignancies is gaining popularity in both basic and clinically driven research. But to date, the majority of such analyses have used microarrays and quantitative real-time PCR. With the introduction of digital count technologies, such as next-generation sequencing (NGS) and the NanoString nCounter System, we have at our disposal, many more options. To make effective use of these different platforms, the strengths and pitfalls of several miRNA profiling technologies were assessed, including a microarray platform, NGS technologies and the NanoString nCounter System. These results were compared to gold-standard quantitative real-time PCR. Comparison of non-small cell lung cancer cell lines grown in vitro (n = 5) and in vivo (n = 5) as xenograft models.

ORGANISM(S): Homo sapiens

SUBMITTER: Shirley Tam 

PROVIDER: E-GEOD-51507 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

Robust global microRNA expression profiling using next-generation sequencing technologies.

Tam Shirley S   de Borja Richard R   Tsao Ming-Sound MS   McPherson John D JD  

Laboratory investigation; a journal of technical methods and pathology 20140120 3

miRNAs are a class of regulatory molecules involved in a wide range of cellular functions, including growth, development and apoptosis. Given their widespread roles in biological processes, understanding their patterns of expression in normal and diseased states will provide insights into the consequences of aberrant expression. As such, global miRNA expression profiling of human malignancies is gaining popularity in both basic and clinically driven research. However, to date, the majority of su  ...[more]

Similar Datasets

Transcriptomics Comparison of microRNA Profiling Platforms (NanoString)
2013-10-22 | E-GEOD-51504 | biostudies-arrayexpress
Transcriptomics Comparison of microRNA Profiling Platforms (RT-PCR)
2013-10-22 | E-GEOD-51503 | biostudies-arrayexpress
Transcriptomics Comparison of microRNA Profiling Platforms (BeadChip)
2013-10-22 | E-GEOD-51501 | biostudies-arrayexpress
Genomics Comparison of microRNA Profiling Platforms (HTS)
2013-10-22 | GSE51507 | GEO
Other Comparison of microRNA Profiling Platforms (NanoString)
2013-10-22 | GSE51504 | GEO
Other Comparison of microRNA Profiling Platforms (RT-PCR)
2013-10-22 | GSE51503 | GEO
Genomics Comparison of microRNA Profiling Platforms (BeadChip)
2013-10-22 | GSE51501 | GEO
Transcriptomics Nanostring nCounter human miRNA assay (v1) of eosinophilic esophagitis
2014-09-01 | E-GEOD-55794 | biostudies-arrayexpress
Transcriptomics Expression signature characteristic of Anaplastic Large Cell Lymphoma (ALCL) patients
2023-12-06 | GSE217426 | GEO
Transcriptomics TRIM28 keeps the time of mitotic gene expression sustaining proliferation and progression of Malignant Pleural Mesothelioma (MPM).
2022-08-03 | GSE183088 | GEO