Niche-independent high-purity cultures of Lgr5+ intestinal stem cells and their progeny

ABSTRACT: Although Lgr5+ intestinal stem cells have been expanded in vitro as organoids, homogeneous culture of these cells has not been possible so far. Here we show that two small molecules, CHIR99021 and valproic acid, synergistically maintain self-renewal of mouse Lgr5+ intestinal stem cells, resulting in nearly homogeneous cultures. The colony-forming efficiency of cells from these cultures is ~100-fold greater than that of cells cultured in the absence of CHIR99021 and valproic acid, and multilineage differentiation ability is preserved. We made use of these homogeneous cultures to identify conditions employing simultaneous modulation of Wnt and Notch signaling to direct lineage differentiation into mature enterocytes, goblet cells and Paneth cells. Expansion in these culture conditions may be

ORGANISM(S): Mus musculus

SUBMITTER: Henner Farin 

PROVIDER: E-GEOD-51539 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress