Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Unravelling osmotololerance in Escherichia coli using a pseudosexual model system

ABSTRACT: Transcriptional analysis of mutants evolved for tolerance to excess sodium chloride (up to 0.75 M) Mutant compared to parental reference during sustained exponential growth in 0.55 M NaCl, two biological replicates per sample, reference is unevolved parent strain for each mutant

ORGANISM(S): Escherichia coli BW25113

SUBMITTER: James Winkler

PROVIDER: E-GEOD-51611 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Evolved osmotolerant Escherichia coli mutants frequently exhibit defective N-acetylglucosamine catabolism and point mutations in cell shape-regulating protein MreB.

Winkler James D JD Garcia Carlos C Olson Michelle M Callaway Emily E Kao Katy C KC

Applied and environmental microbiology 20140411 12

Biocatalyst robustness toward stresses imposed during fermentation is important for efficient bio-based production. Osmotic stress, imposed by high osmolyte concentrations or dense populations, can significantly impact growth and productivity. In order to better understand the osmotic stress tolerance phenotype, we evolved sexual (capable of in situ DNA exchange) and asexual Escherichia coli strains under sodium chloride (NaCl) stress. All isolates had significantly improved growth under selecti ...[more]

PMID: 24727267

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Dataset Information

Unravelling osmotololerance in Escherichia coli using a pseudosexual model system

Publications

Evolved osmotolerant Escherichia coli mutants frequently exhibit defective N-acetylglucosamine catabolism and point mutations in cell shape-regulating protein MreB.

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