Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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D. nodosus grown in the presence/absence of ground hoof


ABSTRACT: This study aimed to identify genes that were differentially expressed when D. nodosus was grown on agar media supplemented with ground hoof compared to the standard agar media. Bacteria were grown on EYE agar or EYE agar with 2% ground hoof agar plates for 2 days before Trizol extraction of RNA. Four biological replicates including two dye swaps were analysed. Raw data was compiled using IMAGENE. Normalisation and statistical analysis was undertaken using BASE. Statistics were performed using the Wilcoxon signed ranked test.

ORGANISM(S): Dichelobacter nodosus

SUBMITTER: Dane Parker 

PROVIDER: E-GEOD-5166 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications


Dichelobacter nodosus causes ovine footrot, a disease that leads to severe economic losses in the wool and meat industries. We sequenced its 1.4-Mb genome, the smallest known genome of an anaerobe. It differs markedly from small genomes of intracellular bacteria, retaining greater biosynthetic capabilities and lacking any evidence of extensive ongoing genome reduction. Comparative genomic microarray studies and bioinformatic analysis suggested that, despite its small size, almost 20% of the geno  ...[more]

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