Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

9 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression data from the stomach of mice treated with dexamethasone.

ABSTRACT: Glucocorticoids are used for the treatment of inflammatory conditions but they also cause many side-effects. We used microarrays to detail the changes in gene expression induced in the stomach upon oral glucocorticoid treatment. Female wildtype Balb/c mice were treated for three days with 50 mg/l dexamethasone (dex) via the drinking water. Control mice were left untreated. The stomach was dissected and RNA prepared from the corpus.

ORGANISM(S): Mus musculus

SUBMITTER: Holger Reichardt

PROVIDER: E-GEOD-51669 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Reduced Treg suppressive function and HIF-1Î± expression in hypoxia

Project description:Recently, hypoxia via the transcription factor HIF-1a has been implicated to play an important role for the fate of the adaptive immune response by regulatory T cells (Treg) and T helper 17 cells (TH17) in the mouse model. However, the reports on the effect of HIF-1a are conflicting and so far no functional data in the human system are available. Therefore, we analyzed the effect of hypoxia and HIF-1a on Treg and TH17 in the human system. FACS, western blot and reporter assays clearly demonstrated that hypoxia does not up-regulate the level of HIF-1a in CD4+ T cells (THC) and microarray analysis revealed no change of the transcriptome comparing normoxia vs. hypoxia. Furthermore, we could show that HIF-1a is almost exclusively regulated via NF-kB and NFAT, whereas hydroxylation and subsequent degradation of HIF-1a had little to no effect. In addition, we showed that HIF-1a is essential for nTreg mediated suppression and for IL-17A secretion of TH17, but not for TH17 lineage commitment measured by RORÎ³t expression. In conclusion, our results demonstrated that THC have a distinct regulation of HIF-1a protein levels, which was absolutely essential for Treg and TH17 function. 3 patients, 2 cell type, 2 treatments = 12 arrays

2013-12-31 | E-GEOD-41999 | biostudies-arrayexpress

Behavioural and functional characterization of Kv10.1 (Eag1) knockout mice

Project description:Kv10.1 (Eag1), member of the Kv10 family of voltage-gated potassium channels, is preferentially expressed in adult brain. The aim of the present study was to unravel the functional role of Kv10.1 in the brain by generating knockout mice where the voltage sensor and pore region of Kv10.1 was removed to render non-functional proteins through deletion of exon 7 of the KCNH1 gene using the “3 Lox P strategy”. Kv10.1-deficient mice show no obvious alterations during embryogenesis and develop normally to adulthood; cortex, hippocampus and cerebellum appear anatomically normal. Other tests, including general health screen, sensorimotor functioning and gating, anxiety, social behaviour, learning and memory did not show any functional aberrations in Kv10.1 null mice. Kv10.1 null mice display mild hyperactivity and longer-lasting haloperidol-induced catalepsy, but there was no difference between genotypes in amphetamine sensitisation and withdrawal, reactivity to apomorphine and haloperidol in the prepulse inhibition tests or to antidepressants in the haloperidol-induced catalepsy. Furthermore, electrical properties of Kv10.1 in cerebellar Purkinje cells did not show any difference between genotypes. Bearing in mind that Kv10.1 is overexpressed in over 70% of all human tumours and that its inhibition leads to a reduced tumour cell proliferation, the fact that deletion of Kv10.1 does not show a marked phenotype is a prerequisite for utilising Kv10.1 blocking and/or reduction techniques, such as siRNA, to treat cancer. 4 mice each for control wildtype and knockout mice

2013-02-11 | E-GEOD-44110 | biostudies-arrayexpress

Expression data from the stomach of mice treated with dexamethasone.

Project description:Glucocorticoids are used for the treatment of inflammatory conditions but they also cause many side-effects. We used microarrays to detail the changes in gene expression induced in the stomach upon oral glucocorticoid treatment.

2014-07-18 | GSE51669 | GEO

Time course bulk RNA-seq of Troy+ chief cells in the mouse stomach corpus epithelium after injury

Project description:The purpose of this experiment is to assess temporal transcriptomic change in Troy+ chief cells upon injury in the stomach corpus in mice. Troy+-eGFP cells were sorted at each time point after stomach injury by DMP777 treatment.

2022-05-05 | E-MTAB-10373 | biostudies-arrayexpress

Bulk RNA-seq of proliferating cells in the mouse stomach corpus epithelium from FUCCI mouse.

Project description:The purpose of this bulk RNA-seq experiment is to identify candidate genes that can mark proliferating isthmus progenitors in the mouse stomach corpus epithelium. The cycling isthmus progenitors were isolated based on mVenus expression in FUCCI mouse and used for bulk RNA-seq data generation. The corpus epithelium was stained with E-cadherin and E-cadherin+ cells were also sorted and used for the control.

2019-08-14 | E-MTAB-6850 | biostudies-arrayexpress

Gene expression analysis of human amnion mesenchymal cells treated with dexamethasone

Project description:Analysis of cultured human amnion mesenchymal cells treated with synthetic glucocorticoid dexamethasone for 48 hours. Results provide insight into the molecular response of the human amnion to glucocorticoids.

2022-09-09 | E-MTAB-9493 | biostudies-arrayexpress

miRNA analysis in myofibroblats derived from normal stomach, both antrum (A) and corpus (C) separately and gastric cancers

Project description:We prepared miRNA from myofibroblats derived from normal stomach, both antrum (A) and corpus (C) separately and gastric cancers. miRCURYTM LNA Array ver5 was performed and comparisons were made by the dual (reference) method.

2016-02-01 | GSE76218 | GEO