Altered DNA Methylation Patterns in Human Oral Squamous Cell Carcinoma Cells

ABSTRACT: To gain insight into the DNA methylation changes during OSCC carcinogenesis, we utilized cytosine conversion and next generation sequencing based technique- enhanced reduced representation bisulfite sequencing (ERRBS) - to assess DNA methylation in cultured, human TERT-immortalized, non-tumorigenic OKF6-TERT1R and OSCC SCC-9 cells. OKF6-TERT1R cells and SCC-9 cells were plated in 10 cm2 tissue culture plates at the density of 2 M-CM-^W 106 cells/plate. Total genomic DNA was extracted from OKF6-TERT1R and SCC-9 cells using DNeasy Blood & Tissue Kit (Qiagen, Germantown, MD, USA), including an RNAse treatment step.

ORGANISM(S): Homo sapiens

SUBMITTER: Katarzyna Marcinkiewicz 

PROVIDER: E-GEOD-51752 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress