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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Neuroligin-associated microRNA-932 targets actin and regulates memory

ABSTRACT: Increasing evidence suggests microRNAs (miRNAs) control levels of mRNA expression during development of the nervous system and during sensory elicited remodelling of the brain. We used an associative olfactory learning paradigm (proboscis extension response) in the honeybee Apis mellifera to detect gene expression changes in the brain. Transcriptome analysis of bees trained to associate an odor with a reward and control bees exposed to air without reward, helped us abstract mRNA-miRNA interactions for empirical testing. Functional studies, feeding cholesterol-conjugated antisense RNA to bees resulted in the inhibition of miR-210 and of miR-932 that is embedded within the neuroligin 2 (Nlg2) gene involved in synapse development. Loss of miR-932 prevents long-term memory formation but not learning. We validated 3M-bM-^@M-^YUTR target site interactions of miR-932 and show miR-932 dysregulates actin, a key cytoskeletal molecule involved in neuronal development and activity-dependent plasticity of the brain. The analysis used Air group (no odor learning) as control sample for comparison to two groups of odor-conditioned bees: linalool and floral mix.

ORGANISM(S): Apis mellifera

SUBMITTER: Alexandre Cristino

PROVIDER: E-GEOD-51795 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Neuroligin-associated microRNA-932 targets actin and regulates memory

Project description:Increasing evidence suggests microRNAs (miRNAs) control levels of mRNA expression during development of the nervous system and during sensory elicited remodelling of the brain. We used an associative olfactory learning paradigm (proboscis extension response) in the honeybee Apis mellifera to detect gene expression changes in the brain. Transcriptome analysis of bees trained to associate an odor with a reward and control bees exposed to air without reward, helped us abstract mRNA-miRNA interactions for empirical testing. Functional studies, feeding cholesterol-conjugated antisense RNA to bees resulted in the inhibition of miR-210 and of miR-932 that is embedded within the neuroligin 2 (Nlg2) gene involved in synapse development. Loss of miR-932 prevents long-term memory formation but not learning. We validated 3’UTR target site interactions of miR-932 and show miR-932 dysregulates actin, a key cytoskeletal molecule involved in neuronal development and activity-dependent plasticity of the brain.

2014-02-01 | GSE51795 | GEO

Paradoxical neurobehavioral rescue by cues associated with infant trauma: Amygdala 5-HT and CORT

Project description:We show that infant trauma, as modeled by infant paired odor-shock conditioning, results in later life depressive-like behavior that can be modulated by learned infant cues (i.e., odor previously paired with shock). We have previously shown that this infant attachment odor learning paradigm results in the creation of a new artificial maternal odor that is able to control pup behavior and retain its value throughout development. Here, we assess the mechanism by which this artificial maternal odor is able to rescue depressive-like behavior and show that this anti-depressant like effect results in glucocorticoid and serotonin (5-HT) related changes in amygdala gene expression and is dependent on amygdala 5-HT. Furthermore, increasing amygdala 5-HT and blocking corticosterone (CORT) in the absence of odor mimics the adult rescue effects elicited by the artificial maternal odor, suggesting a mechanism by which odor presentation exerts its repair effects. There are three experimental groups: 1: pups with no infant shock and the adult forced swim test (FST)with no odor; 2. pups with infant odor-shock pairing and the adult forced swim test (FST) with no odor; 3. pups with infant odor-shock pairing and adult forced swim test with infant odor.

2015-02-04 | E-GEOD-58548 | biostudies-arrayexpress

Transitions in infant learning are modulated by dopamine within the amygdala

Project description:Behavioral transitions Young infant rats paradoxically prefer odors paired with shock but older pups learn aversions. This transition is amygdala- and corticosterone-dependent. Microarray results showed downregulated dopaminergic presynaptic function in the amygdala with preference learning. Corticosterone injected 8-day-old pups and untreated 12-day-old pups learn aversions and had dopaminergic upregulation in the amygdala. 8 day saline or corticosterone treated- or 12 day old untreated rat pups were trained with odor-shock pairings. Immediately after training pups were sacrificed and the amygdala dissected out. Controls were unpaired shock-odor groups.Paired and unpaired groups were processed together for each experimental condition. Paired and unpaired data were compared by ranked products.

2009-09-27 | E-GEOD-17651 | biostudies-arrayexpress

Multi-omics analysis identifies ALDH7A1 as a key regulator of the visual-olfactory bimodal learning in honeybee

Project description:Specific genes or encoded proteins are involved in regulating various learning models of different species through certain signaling pathways，but whether there are also regulatory genes during bimodal learning and memory is largely unknown. Using a multi-omics approach to examine gene expression changes in bees brain performed with three different learning assays, a general up-regulation of genes and proteins were observed in bimodal learning compared to controls. Protein-protein network predictions of differential proteins together with FISH assays suggest ALDH7A1 may be involved in regulation of bimodal learning and memory. Injecting siRNA-ALDH7A1 to the bee brain results in significant inhibition the expressions of ALDH7A1 and regucalcin, and increase β-alanine content. Interestingly, we found that loss of ALDH7A1 only affect visual-olfactory bimodal learning and memory, but not single visual or olfactory conditioned learning after ALDH7A1-RNAi in bees. Therefore, our data suggests that ALDH7A1 may affect bimodal learning and memory though controlling β-alanine related plasticity mechanisms.

2021-09-13 | PXD017008 | Pride

Transcription profiling by array of the brains of bees showing scout and non-scout behaviour

Project description:Scouts and non-scouts (recruits) were collected by using a novelty-seekingM-^] assay. Experiment was conducted in a large outdoor screened enclosure, which enabled us to exert complete control over the location and number of food resources while at the same time studying naturalistic honey bee foraging behavior. Foragers were first trained to a color-marked training feederM-^] that contained unscented 50% sucrose solution (m/v); this initially was the only food source available to them. After 2-3 days of training, a novel feederM-^] was set up in another location in the enclosure, with different color markings and an odor cue. The training feeder was maintained, providing the bees with two possible foraging locations, a familiar and a novel. Scouts were identified as bees that switched foraging from the training feeder to the novel feeder; only bees seen foraging at the novel feeder two or more times and at least once at the training feeder were collected as scouts. Non-scouts (recruits) were collected at the end of the experiments; these were bees that continued to forage at the training feeder, and were never observed to switch to the novel feeder.

2011-07-01 | E-MTAB-491 | biostudies-arrayexpress

Memory consolidation in honey bees is enhanced by down-regulation of Down Syndrome Cell Adhesion Molecule and changes its alternative splicing

Project description:Down syndrome cell adhesion molecule (Dscam) gene encodes a cell adhesion molecule required for neuronal wiring. A remarkable feature of invertebrate Dscam is massive alternative splicing generating thousands of different isoforms from three variable clusters of alternative exons. Dscam expression and diversity arising from alternative splicing have been studied during development, but whether they exert functions in differentiated brains has not been determined. Here, using honey bees, we find that Dscam expression is critically linked to memory retention as reducing expression by RNAi enhances memory after reward learning in adult worker bees. Moreover, alternative splicing of Dscam is altered in all three variable clusters after learning. Since identical Dscam isoforms engage in homophilic interactions, these results suggest a mechanism to alter inclusion of variable exons during memory consolidation possibly to alter neuronal connections for memory retention.

2024-01-05 | GSE244051 | GEO

Transcription profiling by array of honey bee brains from bees that show Varroa Sensitive Hygiene (VSH) behavior

Project description:Expression profiling of bees that have been selected for their high tendency to perform VSH behavior (VSH+) versus control bees, who performs normal VSH behavior (VSH-)

2010-12-01 | E-TABM-1002 | biostudies-arrayexpress

Paradoxical neurobehavioral rescue by cues associated with infant trauma: Amygdala 5-HT and CORT

2015-02-04 | GSE58548 | GEO

Effects of glutamate release inhibition on the transcriptome of BA-NAc neurons

Project description:Reduced reward interest/learning and reward-to-effort valuation are distinct, common symptoms in neuropsychiatric disorders for which chronic stress is a major aetiological factor. Pyramidal glutamate neurons in the basal amygdala (BA) project to various brain regions including nucleus accumbens (NAc). The BA-NAc neural pathway is activated by reward and aversion, with many neurons being monovalent. In adult male mice, chronic social stress (CSS) led to both reduced discriminative reward learning (DRL) associated with decreased BA-NAc Ca2+ activity, and reduced sucrose reward-to-effort valuation (REV) associated, in contrast, with increased BA-NAc Ca2+ activity. Chronic tetanus toxin inhibition of BA-NAc neurons replicated the CSS-DRL effect whilst causing only a mild REV reduction, whilst chronic DREADDs activation of BA-NAc neurons replicated the CSS effect on REV without affecting DRL. This study provides novel evidence that chronic stress disruption of reward processing involves the BA-NAc neural pathway; the bi-directional effects implicate activity changes in BA-NAc reward (learning) and aversion (effort) neurons, with the net overall direction of stress-induced change in activity dependent on on-going stimulus processing and behaviour.

2022-12-01 | GSE216588 | GEO

Effects of Chronic Social Stress on the transcriptome of BA-NAc neurons

2022-12-01 | GSE216587 | GEO

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