Circulating Tumor Cell Clusters are Oligoclonal Precursors of Breast Cancer Metastasis

ABSTRACT: Clusters of circulating tumor cells (CTC-clusters) are present in the blood of patients with cancer but their contribution to metastasis is not well defined. Here, we first use mouse models to demonstrate that breast cancer cells injected intravascularly as clusters are more prone to survive and colonize the lungs than single cells. Primary mammary tumors comprised of tagged cells give rise to oligoclonal CTC-clusters, with 50-fold increased metastatic potential, compared with single CTCs. Using intravital imaging and in vivo flow cytometry, CTC-clusters are visualized in the tumor circulation, and they demonstrate rapid clearance in peripheral vessels. In patients with breast cancer, presence of CTC-clusters is correlated with decreased progression-free survival. RNA sequencing identifies

ORGANISM(S): Homo sapiens

SUBMITTER: Ben Wittner 

PROVIDER: E-GEOD-51827 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress