ChIP analysis unravels an exceptionally wide distribution of binding sites for the NtcA transcription factor in a heterocyst-forming cyanobacterium.

ABSTRACT: The CRP-family transcription factor NtcA, universally found in cyanobacteria, was initially discovered as a regulator operating N control. It responds to the N regime signaled by the internal 2-oxoglutarate levels, an indicator of the C to N balance of the cells. NtcA-activated canonical promoters bear an NtcA-consensus binding site (GTAN8TAC) centered at about 41.5 nucleotides upstream from the transcription start point. In this study, we have used chromatin immunoprecipitation followed by high-throughput sequencing to identify the whole regulon of NtcA in cells of the filamentous, heterocyst-forming cyanobacterium Anabaena sp. PCC 7120 after the withdrawal of combined N. NtcA has been found to bind to 2,424 target regions in the genome of Anabaena, which have been ascribed to 2,153 genes

ORGANISM(S): Anabaena

SUBMITTER: Silvia Picossi 

PROVIDER: E-GEOD-51865 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress