Clustering of CpG islands constitutes an important determinant of the interphase chromosome 3D organization [4C-Seq]

ABSTRACT: Using 4C-Seq experimental procedure we have characterized, in cultured chicken lymphoid and erythroid cells, genome-wide patterns of spatial contacts of several CpG islands scattered along the chromosome 14. A clear tendency for interaction of CpG islands present within the same and different chromosomes has been observed. Accordingly, preferential spatial contacts between Sp1 binding motifs, and other GC-rich genomic elements including DNA sequence motifs capable to form G-quadruplexes were demonstrated. On the other hand, an anchor placed in gene/CpG islands-poor area was found to form spatial contacts with other gene/CpG islands-poor areas within chromosome 14 and other chromosomes. These results corroborate the two compartments model of interphase chromosome spatial organization and su

ORGANISM(S): Gallus gallus

SUBMITTER: Artem Artemov 

PROVIDER: E-GEOD-51937 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress