Array comparative hybridization (array-CGH) for detection of gene dose alterations in VACTERL association [4x180k Array v.031035]

ABSTRACT: The aim of the study was to screen for pathogenic gene dose alterations in patients and fetal cases with VACTERL association using a 180K oligonucleotide microarray. The experiment was performed by hybridization of genomic DNA from patients, labelled with Cy3 fluorophore, with a mix of DNA from healthy sex-matched controls labelled with Cy5 fluorophore (fetal case FC17 was analysed with sex-mismatch for the control DNA). 36 patients/fetal cases were analyzed using a Oxford Gene Technology (OGT) 180K oligonucleotide platform with whole genome coverage (27 patients were analyzed on version 025990 and 9 patients on version 031035). The raw microarray image files were initially analyzed using the Feature Extraction software from Agilent Technologies. Further data analysis was performed using t

ORGANISM(S): Homo sapiens

SUBMITTER: Johanna Winberg 

PROVIDER: E-GEOD-51956 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress