Global and local perturbation of the tomato microRNA pathway by a trans-activated DICER-LIKE 1 mutant

ABSTRACT: DICER-like 1 (DCL1) is a major player in micro RNA (miRNA) biogenesis and accordingly, its few known loss-of-function mutants are either lethal or display arrested seedling development. Consequently, generation of dcl1 mutants by reverse genetics and functional analysis of DCL1 in late-developing organs are challenging. Here, these challenges were resolved through the unique use of trans-activated RNA interference. Global, as well as organ-specific tomato DCL1 (SlDCL1) silencing was induced by crossing the generated responder line (OP:SlDCL1IR) with the appropriate driver line. Constitutive trans-activation knocked down SlDCL1 levels by ~95%, resulting in severe abnormalities including post-germination growth arrest accompanied by decreased miRNA and 21-nucleotide small RNA levels, but pro

ORGANISM(S): Solanum lycopersicum

SUBMITTER: Tzahi Arazi 

PROVIDER: E-GEOD-51959 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress