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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Affymetric 1.0 ST exon array comparing gene expression of bone marrow derived macrophage lacking beta-catenin with control macrophages

ABSTRACT: Bone marrow derived macrophages of Lysz-Cre;Catnbtm2Kem(fl/fl) mouse were compared with bone marrow derived macrophage of Catnbtm2Kem(fl/fl) control mouse Total RNA extracted from bone marrow derived macrophage

ORGANISM(S): Mus musculus

SUBMITTER: Saeid Amini-Nik

PROVIDER: E-GEOD-52163 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Bone marrow-derived macrophages express distinct transcriptional programs when activated by LPS, IFN-gamma and PAO.

Project description:mRNA microarray analysis of bone marrow derived macrophages treated under four conditions, including Naïve (N). Bone marrow derived macrophages (BMDM) were derived from the bone marrow of mice and cultured in the presence of PAO, IFN-gamma, or lipopolysaccharide (LPS). Profiled groups include Naive, LPS, IFN, PAO. Compared each of the groups (PAO, LPS, IFN) with Naïve group.

2013-08-08 | E-GEOD-48609 | biostudies-arrayexpress

MiR expression profile in M1-polarized bone marrow derived macrophage cells compared with normal mouse aortic tissue.

Project description:The global change of the miR expression profile during atherosclerosis is due to the infiltration of different types of leukocytes into the arterail vessel wall in addition to disease-specific regulation in vascular cells. Monocyte-derived macrophage accumulation in the subintimal region is critical in the formation of atherosclerotic plaques. It is currently unknown which miRs are involved in the atherogenic macrophage response. The comparison of the miR expression profile in LPS/Interferon-gamma activated mouse macrophages with the miR expression in the normal aortic vessel wall was performed to detect macrophage-enriched miRs. This screening may help to identify macrophage-enriched miRs in atherosclerotic vessels that may play a role in the macrophage function during atherogenesis. Bone marrow cells were harvested from femura of 6-8 week old female C57BL/6 mice, re-suspended in DMEM-F12/10% FCS/10% L929-conditioned medium, and cultured for 7 days to differentiate into primary macrophages. F4/80 and CD11b expression was determined by flow cytometry to confirm the macrophage phenotype. Macrophages were stimulated with LPS (100ng/ml, 14 hours) and INF-g (10ng/ml, 6 hours) and the M1 polarization was verified by quantification of mannose receptor C type 1 (MRC1), arginase II (ArgII), inducible nitric oxide synthase (iNOS), and arginase I (ArgI) by qRT-PCR. Total RNA (M1-type macrophages and aorta tissue) was isolated using mirVana microRNA Isolation Kit.

2014-01-01 | E-GEOD-26556 | biostudies-arrayexpress

IL-4 stimulation of Dicer deficient bone-marrow derived macrophages in vitro

Project description:Macrophages were derived from the bone-marrow of 3 x fl/+ Dicer LysCre +/- (wild-type) and 3 x fl/fl Dicer LysCre +/- mice and stimulated with IL-4 (50ng/mL) for 72h. Total RNA was isolated and analyzed by gene array. In this experiment, we derived Dicer deficient bone-marrow macrophages using Dicer fl/+ LysM-Cre by Dicer fl/+ crossed mice to obtain Dicer fl/fl LysM-cre progeny (and Dicer deficient macrophages). Next, we studied the effects of IL-4 stimulation in macrophage with a deficiency in Dicer/microRNAs.

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DNA damage responses activate a multi-functional genetic program in murine bone marrow-derived macrophages

Project description:DNA damage response kinase ATM regulates the genetic program of lymphocytes with phsiologically induced DNA DSBs. In bone marrow-derived macrophages, related kinase DNAPKcs is also responsible for activating DNA damage responses after infection with Listeria monocytogenes. Here we show that both ATM and DNA-PKcs regulate the genetic program of Listeria monocytogenes-infected macrophages. Two independent bone marrow-derived macrophage cultures for each genotype (LysMcre/+ and Scid: Atmc/c: LysMcre/+) were infected with Listeria monocytogenes for 24 hrs at an MOI of 5. RNA was isolated using RNeasy (Qiagen). Gene expression profiling was performed using Illumina MouseRef-8 expression microarrays.

2015-07-03 | E-GEOD-70467 | biostudies-arrayexpress

Temporal and ligand effects on phagosome maturation

Project description:Systems biology analysis of temporally-resolved phagosome proteomes following uptake via key phagocytic receptors Macrophages operate at the forefront of innate immunity, and their discrimination of pathogenic versus “self” particles is critical for a number of responses including efficient pathogen killing, antigen presentation and cytokine induction. In order to efficiently phagocytose and destroy the particles, macrophages express an array of receptors to sense and internalise prey particles. In this manuscript, particles conjugated to seven ligands representing pathogen-associated molecular patterns, immune opsonins or apoptotic cell markers were inoculated to murine bone marrow-derived macrophages (BMDMs) and proteomes of isolated phagosomes were accurately quantified among conditions and across a timecourse. While we identified a clear functional differentiation over the three timepoints, only subtle differences were detected between certain ligand-phagosomes, suggesting that triggering of receptors through a single ligand has only mild effects on phagosome proteome and function. This data shows for the first time a systematic time-course analysis of BMDM phagosomes and how phagosome fate is regulated by the receptors triggered for phagocytosis.

2015-09-10 | PXD000772 | Pride

Transcription profiling of mouse IFNg-primed C57BL/6 and C3H.BL6-sst1 bone-marrow macrophages infected with mycobacterium tuberculosis

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2010-01-07 | E-MTAB-90 | biostudies-arrayexpress

Transcription profiling of bone marrow macrophages (BMM), BMM co-cultured with breast cancer cell line 4T1.2, and RAW macrophage cell line

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2019-11-16 | E-MTAB-4064 | biostudies-arrayexpress

MicroRNAome comparison between macrophages infected with Leishmania RNA virus containing L.guyanensis and virus-free parasites

Project description:Leishmania RNA virus is an endosymbiotic virus of obligate intracellular Leishmania parasites. The presence of Leishmania RNA virus has been associated to metastatic leishmaniasis in hamsters and the failure of the first-line treatment in humans. This experiment aims to find the differences in the microRNA profile of bone-marrow derived macrophages infected with Leishmania RNA virus containing L.guyanensis or virus-free parasites.

2016-09-01 | E-MTAB-4413 | biostudies-arrayexpress

Affymetric 1.0 ST exon array comparing gene expression of bone marrow derived macrophage lacking beta-catenin with control macrophages

Project description:Bone marrow derived macrophages of Lysz-Cre;Catnbtm2Kem(fl/fl) mouse were compared with bone marrow derived macrophage of Catnbtm2Kem(fl/fl) control mouse

2014-02-01 | GSE52163 | GEO

MiR expression profiles in M1-polarized compared with unstimulated (MÐ¤) bone marrow-derived macrophage cells

Project description:The global change of the miR expression profile during atherosclerosis is due to the infiltration of different types of leukocytes into the arterial vessel wall in addition to disease-specific regulation in vascular cells. Monocyte-derived macrophage accumulation in the subintimal region is critical in the formation of atherosclerotic plaques. It is currently unknown which miRs are involved in the atherogenic macrophage response. The comparison of the miR expression profile in LPS/Interferon-gamma activated mouse macrophages with the miR expression in the unstimulated mouse macrophages was performed to detect M1-type macrophage-enriched miRs. This screening combined with our miR profiling in atherosclerotic vessels may help to identify M1-type macrophage-enriched miRs in atherosclerotic vessels that may play a role in the macrophage function during atherogenesis. Bone marrow cells were harvested from femura of 6- to 8-week-old female C57BL/6 mice, re-suspended in DMEM-F12/10% FCS/10% L929-conditioned medium, and cultured for 7 days to differentiate into primary macrophages. F4/80 and CD11b expression was determined by flow cytometry to confirm the macrophage phenotype. Macrophages were stimulated with LPS (100ng/ml, 14 hours) and INF-g (10ng/ml, 6 hours), and the M1 polarization was verified by quantification of mannose receptor C type 1 (MRC1), arginase II (ArgII), inducible nitric oxide synthase (iNOS), and arginase I (ArgI) by qRT-PCR. Total RNA (M1-type and unstimulated (MÐ¤) macrophages) was isolated using the mirVana microRNA Isolation Kit.

2012-12-26 | E-GEOD-33453 | biostudies-arrayexpress

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