Inhibition of zygotic DNA repair in trout: transcriptome analysis of the offspring

ABSTRACT: Zygotic repair of paternal genome is a key event after fertilization. Spermatozoa accumulate DNA single and double strand breaks during spermatogenesis and can suffer additional damage before fertilization by different factors, including cryopreservation. Fertilization with DNA damaged spermatozoa (DDS) is considered to promote implantation failures and abortions, but also long term effects in the progeny that could be related to a defective repair. Base excision repair (BER) pathway is considered the most active in zygotic DNA repair, but healthy oocytes contain enzymes for all repairing pathways. In this study the effects of the inhibition of the BER pathway in the zygote, were analyzed on the progeny obtained after fertilization with differentially DDS. Massive gene expression (37.394 t

ORGANISM(S): Oncorhynchus mykiss

SUBMITTER: Julien Bobe 

PROVIDER: E-GEOD-52217 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress