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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Expression Data from E. faecalis V583 under exposure to 10ug/ml vancomycin

ABSTRACT: The vancomycin resistant strain V583 was exposed to therapeutical dose of vancomycin in order to understand the cell response to the antibiotic besides the induction of the vanB genes E. faecalis V583 cells were collected at 10 minutes and 30 min after vancomycin addition and without vancomycin, for RNA extraction and hybridization on Affymetrix microarrays. The cells were collected when at 0.4. For each condition were made replicates.

ORGANISM(S): Enterococcus faecalis V583

SUBMITTER: Neuza Teixeira

PROVIDER: E-GEOD-52263 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression Data from E. faecalis V583 under exposure to 10ug/ml vancomycin

Project description:The vancomycin resistant strain V583 was exposed to therapeutical dose of vancomycin in order to understand the cell response to the antibiotic besides the induction of the vanB genes

2014-01-01 | GSE52263 | GEO

Expression data from E. faecalis Fsr mutants during GBAP induction

Project description:To rigorously characterize the Fsr regulon, we compared gene expression in V583 isogenic mutants in Fsr genes and each of the Fsr-regulated protease genes using microarrays and purified GBAP, the quorum-sensing molecule. We used microarrays to identified the genes that are regulated directly by Fsr Quorum sensing system. E. faecalis V583 cells were collected at 0 minutes and 10 minutes after GBAP addition, for RNA extraction and hybridization on Affymetrix microarrays. The cells were collected when the number of the cells are ideal for Quorum sensing activation. This procedure was also made without GBAP addition. For each condition were made replicates.

2013-05-01 | E-GEOD-42036 | biostudies-arrayexpress

Transcriptomics analysis of E. faecalis V583 exposed to subinhibitory concentration of chlorhexidine

Project description:The vancomycin resistant strain V583 was exposed to subinhibitory concentration of chlorhexidine. E. faecalis V583 cells were collected at 15 minutes after chlorhexidine addition and without chlorhexidine, for RNA extraction and hybridization on Affymetrix microarrays.

2017-01-11 | E-MTAB-5181 | biostudies-arrayexpress

Comparative genomic hybridization of E. faecalis ORGR1F transconjugants mated with V583 to determine the amount of DNA transfered between strains

Project description:Comparative genome hybridization of transconjugants of E. faecalis OG1RF mated with V583. The total DNA of transconjugants was compared with wildtype strains to ascertain the amount of DNA that was transferred from E. faecalis V583 to E. faecalis OG1RF.

2009-05-08 | E-MEXP-2135 | biostudies-arrayexpress

The Enterococcus faecalis V583 transcriptional profile during survival within macrophages

Project description:Transcriptional profiling of E. faecalis V583 during intracellular survival compared to V583 grown to mid-log phase (OD 600 = 0.05) in THB + 1% glucose. In this study we report the complete intracellular E. faecalis V583 transcriptome following infection of RAW264.7 macrophages. During intracellular survival, approximately 45% of the V583 genome was differentially regulated including numerous genes involved in the oxidative stress, heat shock and SOS responses. We observed that the E. faecalis-containing phagosome was limited for glycolytic substrates, nucleotides, amino acids and numerous ions necessary for growth and protein function. Approximately 35% of the genes differentially regulated during survival within macrophages were of hypothetical/unknown function, suggesting that the V583 response to phagocytosis involves many previously unstudied loci. Here, we provide the first comprehensive study elucidating the transcriptional response of E. faecalis to phagocytosis, which may provide new targets for future studies. Bacterial RNA was obtained from RAW264.7 macrophage infected with E. faecalis V583 4, 8 and 12 h post-infection and compared to E. faecalis V583 RNA extracted from mid log growth (OD 600 = 0.05). Control cultures were grown in Todd-Hewett Broth supplemented with 1% glucose.

2011-12-21 | E-GEOD-34590 | biostudies-arrayexpress

Transcriptome profiling of tdc cluster delection mutant of Enterococcus faecalis V583

Project description:The aim was to study the transcriptional profiling of the tdc cluster delection mutant E. faecalis V583 Î?tdc (non-tyramine producer) compared to the wild type strain E. faecalis V583 (tyramine producer). We compared the expression profile of the strains grown in M17 medium with glucose as carbon source and suplemented with tyrosine. E. faecalis V583 Î?tdc cells (test) compared with E. faecalis V583 cells (reference). Both strains grown in GM17 medium suplemented 15 mM tyrosine.

2016-02-15 | E-GEOD-77864 | biostudies-arrayexpress

Transcriptional responses induced by pediocin PA-1 exposure of Enterococcus faecalis V583 and a V583 strain expressing pediocin immunity

Project description:Trancriptional response of pediocin exposure of V583 and V583 expressing immunity (petAIM), and the transcriptional response of petAIM compared to both V583 and V583 containing the vector pet.

2013-11-06 | E-MTAB-655 | biostudies-arrayexpress

Cajanin Stilbene Acid Inhibited Vancomycin-Resistant Enterococci By Inhibiting Phosphotransferase System

Project description:In order to determine the mechanism of Cajanin Stilbene Acid inhibiting vancomycin-resistant enterococci, we compared the changes in protein expression of enterococci V583 strain before and after treated by Cajanin Stilbene Acid.

2020-05-27 | PXD017522 | Pride

Enterococcus faecalis V583

Project description:Expression Data from E. faecalis V583 under exposure to 10ug/ml vancomycin

| PRJNA227309 | ENA

Comparative genomic hybridization of several Enterococcus faecalis strains compared to strain V583

Project description:Gene content in various Enterococcus faecalis strains compared to E. faecalis V583. Strains have been compared to the V583 strain by comparative genomic hybridization using genome-wide PCR-based microarrays representing the V583 genome. Genes have been deemed "present" or "divergent" in the various strains.

2007-01-15 | E-MEXP-931 | biostudies-arrayexpress

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