Transcriptional adaptation of L. monocytogenes EGD to oxygen availability

ABSTRACT: Comparison of aerobic and anaerobic transcriptome of L. monocytogenes EGD L. monocytogenes EGD cells used in this study were grown either aerobically or anaerobically to an OD600 = 0.70 - 0.75 and the transcriptome of these two conditions was compared Six biologically independent cultures of each aerobically and anaerobically grown L. monocytogenes EGD were used for total RNA isolation (six RNA sets). 4 array experiments were performed with Cy5-labelled cDNA derived from aerobically grown Listeria and Cy3-labelled cDNA derived from anaerobcially grown cells. For the two other RNA sets a dye swap was performed, that means the two other array experiments were performed with Cy3-labelled cDNA derived form aerobically grown Listeria and Cy5-labelled cDNA from anaerobically grown cells. As all

ORGANISM(S): Listeria monocytogenes EGDe

SUBMITTER: Stefanie MM-CM-<ller-Herbst 

PROVIDER: E-GEOD-52325 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress