Fine mapping of genome activation in bovine embryos by RNA sequencing

ABSTRACT: During maternal-to-embryonic transition control of embryonic development gradually switches from maternal RNAs and proteins stored in the oocyte to gene products generated after embryonic genome activation (EGA). Detailed insight into the onset of embryonic transcription is obscured by the presence of maternal transcripts. Using the bovine model system, we established by RNA sequencing a comprehensive catalogue of transcripts in germinal vesicle and metaphase II oocytes, and in embryos at the 4-cell, 8-cell, 16-cell and blastocyst stages. These were produced by in vitro fertilization of Bos t. taurus oocytes with sperm from a Bos t. indicus bull to facilitate parent-specific transcriptome analysis. Transcripts from 12.4 to 13.7 M-CM-^W 10^3 different genes were detected in the various deve

ORGANISM(S): Bos taurus

SUBMITTER: Alexander Graf 

PROVIDER: E-GEOD-52415 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress