Parallel analysis of RNA ends enhances global investigation of microRNAs and target RNAs of Brachypodium distachyon

ABSTRACT: The wild grass Brachypodium distachyon has emerged as a model system for temperate grasses and biofuel plants. However, the global analysis of miRNAs, molecules known to be key for eukaryotic gene regulation, has been limited in B. distachyon to studies examining a few samples or that rely on computational predictions. Similarly an in-depth global analysis of miRNA-mediated target cleavage using Parallel Analysis of RNA Ends (PARE) data is lacking in B. distachyon. B. distachyon small RNAs were cloned and deeply sequenced from 17 libraries that represent different tissues and stresses. Using a computational pipeline, we identified 116 miRNAs including not only conserved miRNAs that have not been reported in B. distachyon, but also non-conserved miRNAs that were not found in other plants. T

ORGANISM(S): Brachypodium distachyon

SUBMITTER: Dong-Hoon Jeong 

PROVIDER: E-GEOD-52441 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress