Transcriptome analysis reveals differential splicing events in IPF lung tissue

ABSTRACT: Objectives: Idiopathic pulmonary fibrosis (IPF) is a complex disease in which a multitude of proteins and networks are disrupted. Interrogation of genome-wide transcription through RNA sequencing (RNA-Seq) enables the determination of genes whose differential expression is most significant in IPF, as well as the detection of alternative splicing events which are not easily observed with traditional microarray experiments. Methods: Messenger RNA extracted from 8 IPF lung samples and 7 healthy controls was sequenced on an Illumina HiSeq. Analysis of differential expression and exon usage was performed using Bioconductor packages. The gene periostin was selected for validation of alternative splicing by quantitative PCR, and pathway analysis was performed to determine enrichment for different

ORGANISM(S): Homo sapiens

SUBMITTER: Tracy Nance 

PROVIDER: E-GEOD-52463 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress