Epigenetic and transcriptional landscapes of Dnmt3a-deficient olfactory sensory neurons

ABSTRACT: During differentiation, neurons experience a reorganization of DNA modification patterns within their genomes. However, the mechanisms underlying this developmental patterning and its role in defining the neurona­­l state are currently unclear. Here, we find that the d­­e novo DNA methyltransferase Dnmt3a is necessary for elevated levels of 5-hydroxymethylcytosine (5hmC), a derivative of 5-methylcytosine (5mC), in olfactory sensory neurons (OSNs). Through an analysis of genome-wide 5mC and 5hmC distributions in isolated OSNs, we find that Dnmt3a-dependent 5mC and 5hmC occurs within regions of high accessibility, neural enhancers, and the transcription start sites of transcribed genes. Its loss results in the global disruption of gene expression patterns, including the upregulation of s

ORGANISM(S): Mus musculus

SUBMITTER: Stavros Lomvardas 

PROVIDER: E-GEOD-52464 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress