Lsh regulates LTR retrotransposon repression independent of Dnmt3b function (HELP-seq)

ABSTRACT: Background: Global DNA methylation contributes to genomic integrity by supressing repeat associated transposition events. Several chromatin factors are required in addition to DNA methyltransferases to maintain DNA methylation at intergenic and satellite repeats. Embryos lacking Lsh, a member of the SNF2 superfamily of chromatin helicases, are hypomethylated. The interaction of Lsh with the de novo methyltransferase, Dnmt3b, facilitates the deposition of DNA methylation at stem cell genes. We wished to determine if a similar targeting mechanism operates to maintain DNA methylation at repetitive sequences. Results: We used HELP-seq to map genome wide DNA methylation patterns in Lsh-/- and Dnmt3b-/- somatic cells. DNA methylation is predominantly lost from specific genomic repeats in Lsh-/-

ORGANISM(S): Mus musculus

SUBMITTER: Donncha Dunican 

PROVIDER: E-GEOD-52476 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress