Screening for novel immungenic proteins of Klebsiella pneumoniae MGH 78578

ABSTRACT: The screening of a cDNA derived expression library of Klebsiella pneumoniae MGH 78578 expressed in E.coli using a fusion construct and specific HaloTag interaction to a modified surface is shown. Thus, 1536 different clones were screened including positive (ompA, mdh) and negative (pyrC, gapA) reference proteins. The goal of the screening was to identify potential novel immunogenic proteins from K. pneumoniae by selecting clones showing a high signal intensity in comparison to the known antigens used as positve markers. Afterwards, the most promising clones were sequenced to identify the gene and corresponding protein and these proteins were then investigated further. Consequently, 14 novel immunogenic proteins could be identified. In total 1536 (4 x 384) different lysates were spotted on

ORGANISM(S): Klebsiella pneumoniae

SUBMITTER: Sebastian Hoppe 

PROVIDER: E-GEOD-52536 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress