Epitope Mapping of potential novel immunogenic proteins of Klebsiella pneumoniae MGH 78578

ABSTRACT: A number of six proteins were selected during immunoscreening and further analyses. The proteins were divided in silico into overlapping 15-mer oligopeptides with an overlap of 11 residues. The microarrays were incubated with different antibodies to K. pneumoniae, C. jejuni and S. aureus. Each microarray was seperated into three individual incubation chambers using ProPlate 3-Well modules. Within each incubation chamber each peptide was spotted in triplicate with the controls spotted nine times each. The controls included Human-IgG, Rabbit-IgG, Mouse-IgG and Myelin Basal Protein. Each chamber was incubated independently using different polyclonal antibodies to K. pneumoniae and for specificity testing with antibodies to C. jejuni or S. aureus. Thus, samples 2_1, 2_2, 3_1 and 3_2 represent

ORGANISM(S): Klebsiella pneumoniae

SUBMITTER: Sebastian Hoppe 

PROVIDER: E-GEOD-52537 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress