An RNA-Seq transcriptome and splicing database of neurons, glia, and vascular cells of the cerebral cortex

ABSTRACT: Purpose: To better understand the function of the various cell types of the brain, we prospectively purified neurons, astrocytes, oligodendrocyte precursor cells, newly formed oligodendrocytes, myelinating oligodendrocytes, microglia, endothelial cells, and pericytes from mouse cerebral cortex. We generated a transcriptome database for these 8 cell types by RNA sequencing and used a highly sensitive algorithm to detect alternative splicing events in each gene. Our analysis identified thousands of new cell type enriched genes and splicing isoforms that will provide novel markers for cell identification, new tools for genetic manipulation, and numerous insights into the biology of the brain. Method Part1:To purify astrocytes, we took advantage of a BAC transgenic mouse line expressing EGFP u

ORGANISM(S): Mus musculus

SUBMITTER: kenian chen 

PROVIDER: E-GEOD-52564 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress