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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Overexpression of UHRF1 drives DNA hypomethylation and hepatocellular carcinoma

ABSTRACT: UHRF1 is an essential regulator of DNA methylation that is highly expressed in many cancers. Using transgenic zebrafish, cultured cells and human tumors, we demonstrate that UHRF1 is an oncogene. RNAseq was used to assess the variation in gene expression between control and experimental samples. Total small RNA from 2 batches of Tg(fabp10:has.UHRF1-GFP)High and age matched Tg(fabp10:nls-mCherry) control 5 dpf zebrafish livers was purified for preparation of high-throughput sequencing libraries.

ORGANISM(S): Danio rerio

SUBMITTER: Ravi Sachidanandam

PROVIDER: E-GEOD-52605 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:ATF6 is a key regulator of the unfolded protein response. Through use of zebrafish and cultured cells we demonstrate that ATF6 drives fatty liver disease by interaction with fatty acid synthase (FASN). Total small RNA from livers of 5 dpf larval zebrafish were collected: 2 batches of Tg(fabp10:nls-mCherry) control larvae, 2 batches of ethanol-treated Tg(fabp10:nls-mCherry) larvae, and 1 batch of Tg(fabp10:nAtf6-cherry; cmlc2:GFP). Each batch was purified for preparation of high-throughput sequencing libraries.

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2014-07-26 | E-GEOD-59608 | biostudies-arrayexpress

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Conserved Function of lincRNAs in Vertebrate Embryonic Development Despite Rapid Sequence Evolution

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