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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression in INA6 multiple myeloma cells in an acidic condition

ABSTRACT: We found the epigenetic regulation of gene expression in MM cells in acidic conditions. We further looked into gene expression in MM cells in acidic conditions using a cDNA microarray. INA6 cells were cultured for 12 hours, at pH7.4 or pH6.8 in the absence or presence of valproiate at 100 ug/mL. The total RNA was isolated using RNeasy Mini kit.

ORGANISM(S): Homo sapiens

SUBMITTER: Ryota Amachi

PROVIDER: E-GEOD-52611 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression in INA6 multiple myeloma cells in an acidic condition

Project description:We found the epigenetic regulation of gene expression in MM cells in acidic conditions. We further looked into gene expression in MM cells in acidic conditions using a cDNA microarray.

2013-11-22 | GSE52611 | GEO

Human hepatic stellate cell line LX-2 activated under acidic culture condition

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Expression and differential expression analysis in INA6 cells

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Mycobacterium tuberculosis transcriptional responses to acidic pH and carbon source

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2014-07-07 | E-GEOD-52020 | biostudies-arrayexpress

Expression analysis of INA6 cells after IL-6 withdrawal using TAS

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Maternal peripheral blood natural killer cells incorporate placenta-associated microRNAs during pregnancy

Project description:Although recent studies have revealed that microRNAs (miRNAs) regulate fundamental Natural Killer (NK) cell processes including cytotoxicity and cytokine production, little is known about the miRNA-gene regulatory relationships in maternal peripheral blood NK (pNK) cells during pregnancy. To predict the role of miRNAs within gene regulatory networks of maternal pNK cells during pregnancy, we performed comprehensive miRNA and gene expression profiling of maternal pNK cells using a combination of real-time PCR-based array and DNA microarray analyses and analyzed these differential expression levels between first- and third-trimester pNK cells. Furthermore, we constructed regulatory networks for miRNA-mediated gene expression in pNK cells during pregnancy by Ingenuity Pathway Analysis. By PCR-based array analysis of miRNAs, 12 miRNAs including 6 placenta-derived miRNAs [chromosome 19 microRNA cluster (C19MC) miRNAs] were significantly upregulated in third-trimester pNK cells compared to first-trimester pNK cells. pNK cells incorporated C19MC miRNAs, whose interaction would be mediated via exosomes. Rapid clearance of C19MC miRNAs also occurred in NK cells after delivery. By DNA microarray analysis, 13 NK cell function-related genes were significantly downregulated between first- and third-trimester pNK cells. By pathway and network analysis, 9 downregulated NK-function-associated genes were in silico target candidates of 12 upregulated miRNAs including C19MC miRNA miR-512-3p. The results suggest that transfer of placental C19MC-miRNAs into maternal pNK cells occurs during pregnancy. The present study provides clues to understand maternal NK cell functions Gene expressions in human maternal peripheral blood NK cells were measured at 1st-trimester, 3rd-trimester. Five independent experiments were performed at each term (1st-trimester or 3rd-trimester) using different donors for each experiment.

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