A growth differentiation factor 9 (GDF9) motif regulates follicle-stimulating hormone b-subunit (FSHb) gene expression: involvement of activin receptor-like kinase receptors 4/5 (ALK4/5) in the induction of FSHb by GDF9

ABSTRACT: Gonadotropin-releasing hormone (GnRH) is secreted in brief pulses from the hypothalamus. GnRH regulates follicle-stimulating hormone b-subunit (FSHb) gene expression in pituitary gonadotropes in a frequency-sensitive manner that is central to reproductive physiology. The mechanisms underlying the preferential and paradoxical induction of FSHb by low frequency GnRH pulses are incompletely understood. Here, we identify growth differentiation factor 9 (GDF9) as a novel autocrine inducer of FSHb gene expression. GDF9 gene expression was preferentially suppressed by high frequency GnRH pulses due to reduced transcription. Exogenous GDF9 induced FSHb mRNA expression and knockdown or immunoneutralization of GDF9 reduced FSHb gene expression. Treatment with GDF9 stimulated Smad2/3 phosphorylation

ORGANISM(S): Mus musculus

SUBMITTER: Soon Gang Choi 

PROVIDER: E-GEOD-52631 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress