UVB induces a major genome-wide rearrangement of RNA polymerase II at transcribed human genes

ABSTRACT: Abstract Background: Faithful transcription of DNA is constantly threatened by different endogenous and environmental genotoxic effects. Transcription coupled repair has been described to quickly remove DNA lesions from the transcribed strand of active genes, permitting rapid resumption of blocked transcription. This repair mechanism has been well characterized in the past using individual target genes. However, the precise mechanism by which RNA polymerase II (Pol II) transcription is affected following UV irradiation during the repair processes genome-wide is not well understood. Results: We investigated the effect of a non-lethal dose of UVB on global DNA-bound Pol II distribution in human cells. We find that about 90% of the promoters of expressed genes show reduced Pol II occupancy

ORGANISM(S): Homo sapiens

SUBMITTER: Akos Gyenis 

PROVIDER: E-GEOD-52789 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress