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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Expression data in splenic DC subsets in wild type and Xbp1 deficient mice

ABSTRACT: Xbp1 is a major transcription factor in the unfolded protein response. To uncover its function in DCs we generated a conditional KO for Xbp1 in dendritic cells. We here compare the expression of mRNAs in two different splenic DC subpopulations, CD8a and CD11b DCs in both WT and KO mice. Reference: Inositol-requiring enzyme 1-alpha regulates CD8a dendritic cell function via regulated mRNA decay. Osorio et al, Nature Immunology (2014) Primary DC subsets were isolated and sorted from spleens from 3 different WT or CD11c-cre Xbp-1fl/fl mice. RNA was isolated, converted to cDNA and then hybridised on Affymetrix GeneChip Mouse Gene 1.0 ST Arrays (GPL6246).

ORGANISM(S): Mus musculus

SUBMITTER: Sophie Janssens

PROVIDER: E-GEOD-52857 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The unfolded-protein-response sensor IRE-1α regulates the function of CD8α+ dendritic cells.

Osorio Fabiola F Tavernier Simon J SJ Hoffmann Eik E Saeys Yvan Y Martens Liesbet L Vetters Jessica J Delrue Iris I De Rycke Riet R Parthoens Eef E Pouliot Philippe P Iwawaki Takao T Janssens Sophie S Lambrecht Bart N BN

Nature immunology 20140119 3

The role of the unfolded protein response (UPR) and endoplasmic reticulum (ER) stress in homeostasis of the immune system is incompletely understood. Here we found that dendritic cells (DCs) constitutively activated the UPR sensor IRE-1α and its target, the transcription factor XBP-1, in the absence of ER stress. Loss of XBP-1 in CD11c+ cells led to defects in phenotype, ER homeostasis and antigen presentation by CD8α+ conventional DCs, yet the closely related CD11b+ DCs were unaffected. Whereas ...[more]

PMID: 24441789

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