C10ORF10/DEPP, a transcriptional target of FOXO3 regulates ROS-sensitivity by destabilizing peroxisomes in human neuroblastoma

ABSTRACT: FOXO transcription factors control cellular formation of reactive oxygen species (ROS), which critically contribute to cell survival and cell death in neuroblastoma. Here, we report that C10orf10, also named M-bM-^@M-^\Decidual Protein induced by Progesterone (DEPP)M-bM-^@M-^], is a direct transcriptional target of FOXO3 in human neuroblastoma. As FOXO3-mediated apoptosis involves a biphasic ROS accumulation, we analyzed cellular ROS levels in DEPP-knockdown cells by live-cell imaging. Knockdown of DEPP prevented the primary and secondary ROS accumulation during FOXO3 activation and attenuates FOXO3-induced apoptosis, whereas its overexpression raises cellular ROS levels and sensitizes to cell death. In neuronal cells, cellular steady state ROS are mainly detoxified in peroxisomes by the e

ORGANISM(S): Homo sapiens

SUBMITTER: Johannes Rainer 

PROVIDER: E-GEOD-53046 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress