Replication timing of control and overexpressed PolQ fibroblast cells

ABSTRACT: The human A-family DNA polymerase M-NM-8 (Pol q) is a large, multidomain enzyme whose physiological function is still unclear despite its in vitro translesion synthesis capacity in front of DNA damage and its involvement in some features of DNA repair after external stress. Here we present evidence that Pol q holds a novel role in the absence of external stress as a critical determinant of the replication timing program in human cells. Pol q binds to chromatin at early G1 and is required for proper formation of pre-replicative complexe and replication origin activation. Pol q-depleted cells show modified spatial organization of chromatin-loop structures at replication factories. Genome-wide analysis of replication timing shows delayed replication of a part of early replicating domains and

ORGANISM(S): Homo sapiens

SUBMITTER: Jean-Charles Cadoret 

PROVIDER: E-GEOD-53070 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress