Perturbed gastrulation in embryos from diabetic pregnancies.

ABSTRACT: Maternal diabetes is a teratogen that can lead to neural tube closure defects in the offspring. In neurulation-stage embryos from diabetic dams, we detected abnormal tissue protruding from the open neural tube. To determine the origin of such protrusions, we compared gene expression profiles between open neural plate with normal morphology, and protrusion tissue. Neurulation-stage mouse embryos at 8.5 days of gestation were used to prepare open neural tube at the anterior aspect of neural tube closure site 1 by laser capture microdissection. For each sample, 10 sections were pooled, total RNA was extracted, and 7 ng of total RNA were used for expression profiling by Tag sequencing using an Applied Biosystems SolidSAGE kit for library construction, and an AB SOLiD 5500 XL instrument for seq

ORGANISM(S): Mus musculus

SUBMITTER: J. Michael Salbaum 

PROVIDER: E-GEOD-53075 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress