Transcription profiling of mouse cerebral cortex and striatum after transient focal ischemia in ovarectomized animals to investigate estrogen response after stroke

ABSTRACT: Estrogen reduces brain injury after experimental cerebral ischemia in part through a genomic mechanism of action. Using DNA microarrays, we analyzed the genomic response of the brain to estradiol, and we identified a transcript, cocaine- and amphetamine-regulated transcript (CART), that is highly induced in the cerebral cortex by estradiol under ischemic conditions. Using in vitro and in vivo models of neural injury, we confirmed and characterized CART mRNA and protein up-regulation by estradiol in surviving neurons, and we demonstrated that i.v. administration of a rat CART peptide is protective against ischemic brain injury in vivo. We further demonstrated binding of cAMP response element (CRE)-binding protein to a CART promoter CRE site in ischemic brain and rapid activation by CART of

ORGANISM(S): Rattus norvegicus

SUBMITTER: Nabil Alkayed 

PROVIDER: E-GEOD-5315 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress